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WITec imaging microscopes are equipped with a Köhler-illumination setup with optimized optical components for generating white-light images of outstanding quality. We offer different options including bright- and dark-field imaging and differential interference contrast (DIC). White-light microscopy is possible in reflection and transmission modes and can feature polarization setups.
Optionally, WITec systems can integrate fluorescence microscopy for correlative approaches.
WITec imaging microscopes can integrate bright-field options in reflection or transmission mode, in addition to other techniques including differential interference contrast (DIC) and dark-field imaging.
All light microscopy techniques in WITec microscopes feature software-controlled focus stacking and image stitching. With focus stacking, sharp images of samples of varying height can be quickly generated from a stack of individual images. Automated image stitching combines a series of individual measurements into a large-area image at the highest resolution.
White-light microscopy in WITec microscopes can optionally be upgraded with polarization modules, ideally suited for applications in geology, biology, chemistry and material science. The independent control of the polarizer (excitation path) and analyzer (detection path) facilitate different polarization illumination setups in parallel and perpendicular orientations.
The white-light microscopy capabilities of WITec systems convince with their exceptional performance. In this example, small inclusions were easily found in a diamond, even though they were located 20 – 50 µm beneath the surface. Confocal Raman imaging at the same position then revealed the inclusions' composition.
Combine Raman microscopy and fluorescence imaging in one instrument. The optional fluorescence setup for WITec microscopes uses laser excitation and features a highly sensitive monochromatic camera for detection. Matching emission filters are available for a large number of compatible wavelength combinations.
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