alpha300 R – Raman Imaging Microscope

alpha300 R – Confocal Raman imaging microscope by WITec

While long recognized as the state-of-the-art imaging system, ongoing development resulting from our innovative spirit keeps the WITec's Raman microscope alpha300 R at the forefront of the technology and sets the benchmark in terms of flexibility, sensitivity, speed and performance. These unique characteristics have established the alpha300 R the preeminent confocal Raman imaging system on the market.

The flexibility of the alpha300 R series allows the system to adapt to all requirements, combine different imaging techniques and to evolve to meet new or expanded needs.

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Key Features

Confocal Raman Imaging with unprecedented performance in speed, sensitivity, and resolution
Hyperspectral image generation with the information of a complete Raman spectrum at every image pixel
Excellent lateral resolution
Outstanding depth resolution ideally suited for 3D image generation and depth profiles
Ultra-fast Raman imaging option with under one millisecond integration time per spectrum
Ultra-high throughput spectroscopic system for highest sensitivity and best performance in spectral resolution
Non-destructive imaging technique: no staining of fixation of the sample required

Application Examples

Confocal Raman image of stress in a diamond film. Color coded from red (compressive strain) to blue (tensile strain).

3D Raman Image of a pollen in crystalline honey. Image parameters: 150 x 150 x 50 pixels = 1,125,000 Raman spectra, scan area: 50 µm x 50 µm x 50µm, integration time per spectrum: 12.2 milliseconds, total acquisition time: 3h 50 min.

Color-coded Raman depth-scan of a structured GaN layer used for semiconductors. Violet: GaN; Green: fluorescence along the edges of the structure; Red: Stress in material.

3D confocal color-coded Raman image of an emulsion of oil (green), alkane (magenta), and water (blue). 30 µm x 30 µm x 11.5 µm, 150 x 150 x 23 pixels, 517,500 Raman spectra, total acquisition time: 23 min.

Specifications

Raman General Operation Modes

Raman spectral imaging: acquisition of a complete Raman spectra at every image pixel
Planar (x-y-direction) and depth scans (z-direction) with manual sample positioning
Image stacks: 3D confocal Raman imaging
Time series
Single point Raman spectrum acquisition
Single-point depth profiling
Fibre-coupled UHTS spectrometer specifically designed for Raman microsopy and applications with low light intensities
Confocal Fluorescence Microscopy
Bright Field Microscopy

Basic Microscope Features

Research grade optical microscope with 6x objective turret
Video system: video CCD camera
LED white-light source for Köhler illumination
Manual sample positioning in x- and y-direction
Fibre coupling

Computer Interface:

WITec software for instrument and measurement control, data evaluation and processing

Raman Optional/Upgradable Operation modes

Additional lasers, several wavelenghts eligible
Additional UHTS-spectrometers (UV, VIS, NIR)
Automated, motorized sample positioning and measuring with piezo-driven scan stages
Automated confocal Raman imaging
Automated multi-area and multi-point measurements
Full automation available: see alpha300 apyron
Ultrafast Raman imaging, optional available
Upgradable for epi-fluorescence applications
Adapter for higher samples
TrueSurface for Raman depth profiling
Autofocus
Additional white-light options: Dark Field Microscopy, Phase Contrast Microscopy, and DIC optional

Ultrahigh-throughput UHTS Spectrometers

Various lens-based, excitation optimized spectrometers (UV, VIS or NIR) available, all specifically designed for Raman microsopy and applications with low light intensities
Fibre-coupled ultrahigh-throughput optical instruments
Superior peak shape conservation

Related Applications

Food Technology Forensics Archaeology & The Arts Coatings & Thin Films Energy Storage Geo Science Life Science Materials Science Nano-Carbon & 2D Materials Pharmaceutics & Cosmetics Polymers Semiconductors & Photovoltaics

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