StrobeLock is an extension for the WITec alpha300 microscope series, which enables time-correlated single photon counting (TCSPC) measurements. TCSPC records the time-resolved luminescence or fluorescence decay after pulsed stimulation. In Time-resolved Luminescence Microscopy (TLM), the intensity decay is recorded at each image pixel. Various parameters describing each decay can be determined, such as single- or even multi-exponential relaxation times. Images can then be color-coded according to any of the extracted parameters, revealing local differences in the emission behavior of a sample. For example, extracting the lifetime of fluorophores allows for Fluorescence Lifetime Imaging (FLIM). Combining the time-resolved data with Raman, AFM or SNOM images yields additional information on the investigated materials.
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