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Polarization-Resolved Microscopy and Spectroscopy Solutions

WITec’s spectroscopy and microscopy systems can be configured with our flexible and automatable polarization modules for advanced structural and molecular analysis approaches. We offer solutions for polarization-resolved measurements in a variety of confocal optical techniques, such as white light imaging and spectroscopic applications.

The freely rotatable linear polarizer and analyzer modules enable you to perform spectroscopic measurements at any desired polarization configuration without sample rotation and/or adjustment of the sample position. Additionally, motorized modules allow for comfortable and precise serial recordings at fixed or flexible angular offsets between the polarizer and analyzer.


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Key Features


The Principle

Waveplates in the polarizer module can rotate the linearly polarized light from the laser in a defined direction (half wave plate, λ/2) or transform it into circularly or elliptically polarized light (quarter wave plates, λ/4). A polarization filter in the analyzer within the detection path filters the light from the sample along a selectable polarization axis before detection.

Excitation path with polarizer
Detection path with analyzer

Polarization-resolved Raman imaging

Using polarization analysis in combination with Raman spectroscopy enables the probing of advanced molecular information of the sample including the symmetry of the respective vibrations. As such, the orientation of different domains, chirality and optical anisotropy can easily be visualized.


Application Examples

Polyethylene unstretched
Polyethylene stretched
Polarization-resolved Raman spectroscopy of polyethylene (PE) revealing the stretching-induced fiber alignment within PE plastic foil. 3D heatmaps visualize the polarization-dependent Raman intensity for several modes, including the symmetrical C-C stretching (1130 rel. cm-1), CH2 bending (1417, 1441 and 1464 rel. cm-1), and C-H stretching (2849 and 2883 rel. cm-1) in the stretched condition (right).
Biotite in parallel polarized white light transmission
Biotite Raman Polar Plots
TrueComponent Analysis of biotite
Polarization-resolved analysis of the highly-oriented biotite crystals in metamorphic rock. Left: Parallel polarized white light transmission image. Middle: Polar plots of Raman mode intensities of the T6O6-ring bending modes in individual biotite grains. Right: TrueComponent Analysis image of different spatial crystal orientations.
Polarized Raman imaging series of Abalone
Raman spectra Abalone
Left: Polarized Raman imaging series of the nacre of an abalone shell acquired with a fixed, perpendicular incident and detection angle (cross-polarized configuration) and a rotation in 5-degree steps, resolving the highly organized structuring of aragonite. Right: Representative Raman spectra of the color-coded areas show characteristic aragonite lattice vibration modes at 153 rel. cm-1 (translational), 206 rel. cm-1 (wobbling) and 1085 rel. cm-1 (CO-stretching).

Literature

Technical Note Polarization-resolved Raman microscopy and spectroscopy


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Related Applications

Coatings & Thin FilmsGeo ScienceLife ScienceNano-Carbon & 2D MaterialsSemiconductors & Photovoltaics