Fluorescence Lifetime Imaging Microscopy (FLIM) determines the average fluorescence lifetime for each image pixel from the time-resolved fluorescence decay after excitation by a pulsed laser. The resulting FLIM image is color-coded according to the lifetime, displaying its spatial distribution in a sample. In combination with other imaging techniques, such as Raman imaging or AFM, FLIM extends the amount of information gained from one sample.
Fluorescence Lifetime Imaging (FLIM) of N,N′‐bis(1‐ethylpropyl)‐3,4,9,10‐perylenebis(dicarboximide) (EPPTC) crystal needles. Left: Total fluorescence intensity; scale bar 5 µm. Right: FLIM; scale bar 5 µm.
Images are a courtesy of Xinping Zhang, Institute of Information Photonics Technology and College of Applied Sciences, Beijing University of Technology.